4-Hydroxy-2-hexenal Antibody: PerCP

4-Hydroxy-2-hexenal Antibody: PerCP

• Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
• Description: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal IgG1
• Specifications: Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
• Product Name Alternative: 4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody
• Synonyms: 4-Hydroxy-2-hexenal Antibody, Clone 5C11.1: PerCP
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Host: Mouse
• Species Reactivity: Species Independent
• Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)
• Target: 4-Hydroxy-2-hexenal
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 5C11.1
• Conjugation: PerCP
• Validated Applications: WB, IHC, ICC/IF, ELISA
• Purification: Protein G Purified
• Limit Of Detection: A 1:1000 dilution of SMC-536 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.