4-Hydroxy-2-hexenal Antibody: Biotin

4-Hydroxy-2-hexenal Antibody: Biotin

• Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.
• Description: Mouse Anti-4-Hydroxy-2-hexenal (4-HHE) Monoclonal IgG2b
• Specifications: Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
• Product Name Alternative: 4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody
• Synonyms: 4-Hydroxy-2-hexenal Antibody, Clone 6F10: Biotin
• CAS Number: 9007-83-4
• UNSPSC: 12352203
• Host: Mouse
• Species Reactivity: Species Independent
• Immunogen: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
• Target: 4-Hydroxy-2-hexenal
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone: 6F10
• Conjugation: Biotin
• Validated Applications: WB, IHC, ICC/IF |ELISA
• Purification: Protein G Purified
• Limit Of Detection: A 1:1000 dilution of SMC-510 was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
• Concentration: 1 mg/ml
• Dilution: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50); optimal dilutions for assays should be determined by the user.
• Weight: 0.1
• Buffer: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Precautions: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• References & Citations: 1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.