4-Hydroxy-2-hexenal Antibody

• Background:

  Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 4-hydroxy-2-hexenal (4-HHE) is a major alpha,beta-unsaturated aldehyde product of n-3 PUFA oxidation resulting from lipid peroxidation. High levels of HHE are found in disease states (1). 4-HHE reacts with histidine residues to form Michael-addition type adducts.

• Description:

  Mouse Anti-4-Hydroxy-2-hexenal Monoclonal IgG1

• Specifications:

  Specific for 4-Hydroxy-2-hexenal. Slight cross-reactivity with 4-Hydroxynonenal. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

• Product Name Alternative:

  4-Hydroxy-2-hexenal (4-HHE) Antibody, 4-Hydroxy-2-hexenal Antibody, 4-HHE Antibody, MG Antibody, MG-modified protein Antibody, 4-Hydroxy-2-hexenal-modified , 4-Hydroxy-2-hexenal Antibody, 4-hydroxy Hexenal Antibody, HHE Antibody, 4-HHE Antibody

• Synonyms:

  4-Hydroxy-2-hexenal Antibody, Clone 5C11.1

• UNSPSC:

  12352203

• Host:

  Mouse

• Species Reactivity:

  Species Independent

• Immunogen:

  Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH)

• Target:

  4-Hydroxy-2-hexenal

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  5C11.1

• Conjugation:

  Unconjugated

• Validated Applications:

  WB, ELISA

• Purification:

  Protein G Purified

• Limit Of Detection:

  A 1:1000 dilution of SMC-536 was sufficient for detection of 4-Hydroxy-2-hexenal in 1 µg of 4-Hydroxy-2-hexenal-BSA conjugate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

• Weight:

  0.012

• Buffer:

  PBS pH7.4, 50% glycerol, 0.09% sodium azide

• Precautions:

  Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

• References & Citations:

  1. Long, EK., Picklo, MJ Sr. (2010) Free Radic Biol Med. 49(1):1-8.

• CAS Number:

  9007-83-4