Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (Monomer, MALS verified)

• Background: TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (GLAPPQHLIRV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GLAPPQHLIRV peptide of the p53.
• Description: Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (HLP-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & RMPEAAPPV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & RMPEAAPPV).
• Specifications: Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & RMPEAAPPV peptide
• Host: HEK 293
• Target: HLA-A*0201 | B2M | p53 (RMPEAAPPV)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (Cat. No. HLP-H82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 36.3 kDa and 11.7 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C