PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein

PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein

• Background: TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (HMTEVVRHC) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and HMTEVVRHC peptide of the p53.
• Description: PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein (HLP-HP2He) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Met 119 (B2M) & Gly 25 - Ile 308 (HLA-A*02:01) & HMTEVVRHC peptide (Accession # P61769-1 (B2M) & AAA59606.1 (HLA-A*02:01) & HMTEVVRHC).
• Specifications: PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein is assembled by biotinylated monomer (HLP-H82Ef) and PE-labeled streptavidin. Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. Biotinylated Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
• Expression Region: Ile 21 - Met 119 (B2M) & Gly 25 - Ile 308 (HLA-A*02:01) & HMTEVVRHC peptide
• Host: HEK 293
• Target: HLA-A*0201 & B2M & p53 (HMTEVVRHC)
• Conjugation: PE Labeled
• Tag: C-10×His
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Bioactivity: Immobilized PE-Labeled Human HLA-A*02:01&B2M&p53 (HMTEVVRHC) Tetramer Protein (Cat. No. HLP-HP2He) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-3 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, 1% BSA, pH7.4
• Additives: Trehalose
• Molecular Weight: 49.3 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C, aviod light