Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (Monomer, MALS verified)

• Background:

  TP53 has been recognized as a tumor suppressor. 50% of cancers carry a TP53 mutation while many others affect other pathway components. High-copy numbers of WT p53 peptide-MHC class I complexes were detected on tumor cells as compared to low copies on normal cells. The Human HLA-A*0201 p53 (GLAPPQHLIRV) complex protein is a complex of HLA-A*0201 of the MHC Class I, B2M, and GLAPPQHLIRV peptide of the p53.

• Description:

  Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (HLP-H82E8) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & RMPEAAPPV peptide (Accession # AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & RMPEAAPPV) .

• Specifications:

  Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein is produced by co-expression of HLA and B2M loaded with p53 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 36.3 kDa and 11.7 kDa. The protein migrates as 40-43 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  AAA59606.1 (HLA-A*02:01) & P61769-1 (B2M) & RMPEAAPPV

• Expression Region:

  Gly 25 - Ile 308 (HLA-A*02:01) & Ile 21 - Met 119 (B2M) & RMPEAAPPV peptide

• Host:

  HEK293

• Target:

  HLA-A*0201 | B2M | p53 (RMPEAAPPV)

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Biotinylated Human HLA-A*02:01&B2M&p53 (RMPEAAPPV) Complex Protein (Cat. No. HLP-H82E8) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  36.3 kDa and 11.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  Human