EIF5B Polyclonal Antibody

CAT: 0749-ABP57085-01Size: 30 µLDry Ice: NoHazardous: No
CAT#:0749-ABP57085-01Size:30 µL
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Background
Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3) . Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B (eukaryotic translation initiation factor 5B) . The gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.
Description
Rabbit Anti-eIF5B Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
EIF5B; IF2; KIAA0741; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2
Gene ID
9669
Accession Number
O60841
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from the C-terminal region of human eIF5B at AA range: 1020-1100
Clonality
Polyclonal
Tag
Eukaryotic translation initiation factor 5B
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
139kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
EIF5B Polyclonal Antibody detects endogenous levels of eIF5B protein.
Observed Molecular Weight
139
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Other Product Names
EIF5B; IF2; KIAA0741; Eukaryotic translation initiation factor 5B; eIF-5B; Translation initiation factor IF-2
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/O60841
Isotype
Rabbit IgG

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