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EIF4B (phospho Ser422) Polyclonal Antibody

CAT: 0749-ABP54968-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP54968-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly- (A) binding protein, increasing its poly- (A) binding activity. Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation. Multiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002. Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity. This phosphorylation is shown to be essential for the translational activity of eIF4B.
Description
Rabbit Anti-eIF4B (phospho Ser422) Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
EIF4B; Eukaryotic translation initiation factor 4B; eIF-4B
Gene ID
3716
Accession Number
P23588
Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from human eIF4B around the phosphorylation site of S422
Clonality
Polyclonal
Tag
Eukaryotic translation initiation factor 4B
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
80kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Phospho-eIF4B (S422) Polyclonal Antibody detects endogenous levels of eIF4B protein only when phosphorylated at S422.
Observed Molecular Weight
80
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.
Other Product Names
EIF4B; Eukaryotic translation initiation factor 4B; eIF-4B
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P23588
Isotype
Rabbit IgG

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