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GUF1 Polyclonal Antibody

CAT: 0749-ABP56056-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP56056-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.
Description
Rabbit Anti-GUF1 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
GUF1; Translation factor GUF1; mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase
Gene ID
60558
Accession Number
Q8N442
Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from the Internal region of human GUF1 at AA range: 390-470
Clonality
Polyclonal
Tag
Translation factor GUF1 mitochondrial
Applications
IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
GUF1 Polyclonal Antibody detects endogenous levels of GUF1 protein.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300; ELISA 1:20000; IF 1:50-200
Other Product Names
GUF1; Translation factor GUF1; mitochondrial; Elongation factor 4 homolog; EF-4; GTPase GUF1; Ribosomal back-translocase
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q8N442
Isotype
Rabbit IgG

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