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ZP4 Polyclonal Antibody

CAT: 0749-ABP55937-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP55937-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, ZP4 (zona pellucida glycoprotein 4) has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.
Description
Rabbit Anti-ZP4 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
ZP4; ZPB; Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zp-4; Zona pellucida protein B
Gene ID
57829
Accession Number
Q12836
Reactivity
Human, Rat
Immunogen
Synthesized peptide derived from the Internal region of human ZP4 at AA range: 200-280
Clonality
Polyclonal
Tag
Zona pellucida sperm-binding protein 4
Applications
WB, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
65kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
ZP4 Polyclonal Antibody detects endogenous levels of ZP4 protein.
Observed Molecular Weight
65
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.
Other Product Names
ZP4; ZPB; Zona pellucida sperm-binding protein 4; Zona pellucida glycoprotein 4; Zp-4; Zona pellucida protein B
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q12836
Isotype
Rabbit IgG

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