E2F-4 Polyclonal Antibody

• Background:

  Transcription factor E2F4 encoded by E2F4 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

• Description:

  Rabbit Anti-E2F-4 Polyclonal Antibody

• Product Name Alternative:

  E2F4; Transcription factor E2F4; E2F-4

• Gene ID:

  1874

• Accession Number:

  Q16254

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from human E2F-4 around the non-acetylation site of K96

• Clonality:

  Polyclonal

• Tag:

  Transcription factor E2F4

• Applications:

  WB, IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  43kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  E2F-4 Polyclonal Antibody detects endogenous levels of E2F-4 protein.

• Observed Molecular Weight:

  43

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC: 1:100-1:300; ELISA 1:20000; IF 1:50-200

• Other Product Names:

  E2F4; Transcription factor E2F4; E2F-4

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/Q16254

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG