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E2F-2 Polyclonal Antibody

CAT: 0749-ABP51213-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP51213-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
E2F transcription factor 2 encoded by E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
Description
Rabbit Anti-E2F-2 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
E2F2; Transcription factor E2F2; E2F-2
Gene ID
1870
Accession Number
Q14209
Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from the Internal region of human E2F-2 at AA range: 190-270
Clonality
Polyclonal
Tag
Transcription factor E2F2
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
48kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
E2F-2 Polyclonal Antibody detects endogenous levels of E2F-2 protein.
Observed Molecular Weight
48
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200
Other Product Names
E2F2; Transcription factor E2F2; E2F-2
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q14209
Isotype
Rabbit IgG

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