BCKDK Polyclonal Antibody

• Background:

  The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein (branched chain ketoacid dehydrogenase kinase) encoded by BCKDK is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for BCKDK.

• Description:

  Rabbit Anti-BCKDK Polyclonal Antibody

• Product Name Alternative:

  BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN

• Gene ID:

  10295

• Accession Number:

  O14874

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthesized peptide derived from the N-terminal region of human BCKDK at AA range: 30-110

• Clonality:

  Polyclonal

• Tag:

  [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial

• Applications:

  WB, IHC, IF, ELISA

• Purification Method:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• Concentration:

  1 mg/ml

• Molecular Weight:

  46kD

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

• Notes:

  BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.

• Formulation:

  Liquid solution

• Buffer pH:

  Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• Applications Notes:

  Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

• Other Product Names:

  BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN

• Tested Applications:

  WB, IHC, IF, IP, ELISA

• Host or Source:

  Rabbit

• Accession Number URL:

  http://www.uniprot.org/uniprot/O14874

• CAS Number:

  9007-83-4

• Isotype:

  Rabbit IgG