Products for Research Use Only

AAK1 Polyclonal Antibody

CAT: 0749-ABP50558-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP50558-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 encodes a member (AP2 associated kinase 1) of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Description
Rabbit Anti-AAK1 Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
Gene ID
22848
Accession Number
Q2M2I8
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Synthesized peptide derived from the Internal region of human AAK1 at AA range: 240-320
Clonality
Polyclonal
Tag
AP2-associated protein kinase 1
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
94kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
AAK1 Polyclonal Antibody detects endogenous levels of AAK1 protein.
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.
Other Product Names
AAK1; KIAA1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q2M2I8
Isotype
Rabbit IgG