Golgin 45 rabbit pAb

Golgin 45 rabbit pAb

• Description: Caution: Because of the presence of a potential basic motif and leucine-zipper domain, PubMed: 9129147 and PubMed: 11056056 have thought that BLZF1 is a potential transcription factor. They found it localized in the nucleus, except isoform 2, which was cytoplasmic. However, homology at several typical position for basic or hydrophobic residues is missing. function: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. induction: Up-regulated by retinoids. subunit: Interacts with GORASP2 and with the GTP-bound form of RAB2, but not with other Golgi Rab proteins. GORASP2 and BLZF1 form a RAB2 effector complex on medial Golgi. tissue specificity: Ubiquitous. Also found in cell lines derived from several hematopoietic pathologies, such as T-cell leukemia, pro-B, pre-B, myeloma, and plasmacytoma cell lines, but not in Burkitt lymphoma cells.
• Synonyms: BLZF1; JEM1; Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor
• Gene ID: 8548
• UniProt: Q9H2G9
• Cellular Locus: Golgi apparatus membrane.; [Isoform 1]: Nucleus. Detected in the nucleus upon heterologous expression. Not detected in the cytoplasm..; [Isoform 2]: Cytoplasm. Not detected in the nucleus..
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human BLZF1. AA range:10-59
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Molecular Weight: 47kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.