Cleaved-Caspase-4/5 p20 (D270/D311) rabbit pAb

• Background:

  This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

• Description:

  This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

• Synonyms:

  CASP4; ICH2; Caspase-4; CASP-4; ICE(rel)-II; Protease ICH-2; Protease TX; CASP5; ICH3; Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY

• Gene ID:

  837

• UniProt:

  P49662/P51878

• Cellular Locus:

  Cytoplasm, cytosol. Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630)..

• Host:

  Rabbit

• Species Reactivity:

  Human

• Reactivity:

  Human

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human Caspase 4/5. AA range:221-270

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA

• Validated Applications:

  WB, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  47,22kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  47,22 kD

• Subcellular Location:

  Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER) . Association with the ER membrane requires TMEM214 (PubMed:15123740) . Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630) . .

• Other Product Names:

  CASP4; ICH2; Caspase-4; CASP-4; ICE (rel) -II; Protease ICH-2; Protease TX; CASP5; ICH3; Caspase-5; CASP-5; ICE (rel) -III; Protease ICH-3; Protease TY

• Gene ID (Human):

  837

• SwissProt (Human):

  P49662/P51878