Caspase 4 (p10, Cleaved-Ala290) rabbit pAb

Caspase 4 (p10, Cleaved-Ala290) rabbit pAb

• Description: Catalytic activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-. function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. similarity: Belongs to the peptidase C14A family. similarity: Contains 1 CARD domain. subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. tissue specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.
• Synonyms: Caspase-4 (CASP-4;EC 3.4.22.57;ICE(rel)-II;Protease ICH-2;Protease TX) [Cleaved into: Caspase-4 subunit 1; Caspase-4 subunit 2]
• Gene ID: 837
• UniProt: P49662
• Cellular Locus: Cytoplasm, cytosol. Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted. Predominantly localizes to the endoplasmic reticulum (ER). Association with the ER membrane requires TMEM214 (PubMed:15123740). Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630)..
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthesized peptide derived from human Caspase 4 (p10, Cleaved-Ala290)
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: WB 1:1000-2000 ELISA 1:5000-20000
• Molecular Weight: 10 42kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.