Frizzled-6 rabbit pAb

• Background:

  Frizzled class receptor 6 (FZD6) Homo sapiens This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]

• Description:

  Frizzled class receptor 6 (FZD6) Homo sapiens This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide. [provided by RefSeq, Aug 2011],

• Synonyms:

  FZD6; Frizzled-6; Fz-6; hFz6

• Gene ID:

  8323

• UniProt:

  O60353

• Cellular Locus:

  Membrane ; Multi-pass membrane protein. Cell membrane ; Multi-pass membrane protein. Cell surface. Apical cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane ; Multi-pass membrane protein. Endoplasmic reticulum membrane ; Multi-pass membrane protein. Colocalizes with FZD3 at the apical face of cells (By similarity). Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity)..

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse,

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human FZD6. AA range:116-165

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, IHC, IF, ELISA

• Validated Applications:

  WB, IHC, IF, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight:

  79kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  79 kD

• Subcellular Location:

  Membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cell surface . Apical cell membrane; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Colocalizes with FZD3 at the apical face of cells (By similarity) . Localizes to the endoplasmic reticulum membrane in the presence of LMBR1L (By similarity) . .

• Other Product Names:

  FZD6; Frizzled-6; Fz-6; hFz6

• Gene ID (Human):

  8323

• SwissProt (Human):

  O60353