RCC2 rabbit pAb

• Background:

  Function:Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 7 RCC1 repeats., subcellular location:Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex., subunit:Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

• Description:

  Function: Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Contains 7 RCC1 repeats. subcellular location: Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex. subunit: Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

• UniProt:

  Q9P258

• Swiss Prot:

  Q9P258

• Reactivity:

  Human; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human RCC2. AA range:473-522

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight:

  56kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  56kD

• Fragment:

  IgG

• Subcellular Location:

  Nucleus, nucleolus . Nucleus . Cytoplasm, cytoskeleton . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome . Midbody . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378) . Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455) . Colocalizes with RAC1 at the cell membrane (PubMed:25074804) . .

• Other Product Names:

  RCC2; KIAA1470; TD60; Protein RCC2; RCC1-like protein TD-60; Telophase disk protein of 60 kDa

• Gene ID (Human):

  55920