MLK1/2 (phospho Thr312/266) rabbit pAb

MLK1/2 (phospho Thr312/266) rabbit pAb

• Description: Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. enzyme regulation: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation. function: Activates the JUN N-terminal pathway. PTM: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. similarity: Contains 1 protein kinase domain. similarity: Contains 1 SH3 domain. subunit: Homodimer. tissue specificity: Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.
• Synonyms: MAP3K9; MLK1; PRKE1; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1; MAP3K10; MLK2; MST; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; Protein kinase MST
• Gene ID: 4293/4294
• UniProt: P80192/Q02779
• Cellular Locus: Intracellular,integral component of membrane,
• Host: Rabbit
• Species Reactivity: Human, Mouse
• Immunogen: The antiserum was produced against synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266. AA range:281-330
• Clonality: Polyclonal
• Validated Applications: IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.