DGK-θ rabbit pAb

• Background:

  The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

• Description:

  The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008],

• UniProt:

  P52824

• Swiss Prot:

  P52824

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human DGKQ. AA range:691-740

• Clonality:

  Polyclonal

• Source:

  Rabbit

• Applications:

  WB; IHC; IF; ELISA

• Concentration:

  1 mg/ml

• Dilution:

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

• Molecular Weight:

  101kD

• Storage Conditions:

  -20°C/1 year

• Observed Molecular Weight:

  101kD

• Fragment:

  IgG

• Subcellular Location:

  Cytoplasm . Cytoplasm, cytosol . Cell membrane . Cell junction, synapse . Cytoplasm, cytoskeleton . Nucleus . Nucleus speckle . Nucleus matrix . Translocates to the plasma membrane in response to steroid hormone receptor stimulation (PubMed:15632189) . Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH (PubMed:15632189) . Translocates to the nucleus in response to thrombin stimulation (Probable) . Association with the nuclear matrix is regulated by nerve growth factor (By similarity) . .

• Other Product Names:

  DGKQ; DAGK4; Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta

• Gene ID (Human):

  1609