DNA pol ι rabbit pAb

DNA pol ι rabbit pAb

• Description: Catalytic activity: Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1). Cofactor: Magnesium. Domain: The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. function: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. similarity: Belongs to the DNA polymerase type-Y family. similarity: Contains 1 umuC domain. subcellular location: Accumulates at replication forks after DNA damage. subunit: Binds REV1L (By similarity). Binds POLH. tissue specificity: Ubiquitous. Highly expressed in testis.
• Synonyms: POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B
• Gene ID: 11201
• UniProt: Q9UNA4
• Cellular Locus: Nucleus. Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage..
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690
• Clonality: Polyclonal
• Validated Applications: WB, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 85kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.