DNA pol ι rabbit pAb

• Background:

  Catalytic activity:Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1) ., cofactor:Magnesium., domain:The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity., function:Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity., similarity:Belongs to the DNA polymerase type-Y family., similarity:Contains 1 umuC domain., subcellular location:Accumulates at replication forks after DNA damage., subunit:Binds REV1L (By similarity) . Binds POLH., tissue specificity:Ubiquitous. Highly expressed in testis.

• Description:

  Catalytic activity: Deoxynucleoside triphosphate + DNA (n) = diphosphate + DNA (n+1). Cofactor: Magnesium. Domain: The catalytic core consists of fingers, palm and thumb subdomains, but the fingers and thumb subdomains are much smaller than in high-fidelity polymerases; residues from five sequence motifs of the Y-family cluster around an active site cleft that can accommodate DNA and nucleotide substrates with relaxed geometric constraints, with consequently higher rates of misincorporation and low processivity. function: Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. similarity: Belongs to the DNA polymerase type-Y family. similarity: Contains 1 umuC domain. subcellular location: Accumulates at replication forks after DNA damage. subunit: Binds REV1L (By similarity). Binds POLH. tissue specificity: Ubiquitous. Highly expressed in testis.

• Synonyms:

  POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

• Gene ID:

  11201

• UniProt:

  Q9UNA4

• Cellular Locus:

  Nucleus. Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage..

• Host:

  Rabbit

• Species Reactivity:

  Human, Rat, Mouse,

• Reactivity:

  Human; Rat; Mouse

• Immunogen:

  The antiserum was produced against synthesized peptide derived from human POLI. AA range:641-690

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA

• Validated Applications:

  WB, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight:

  85kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  85 kD

• Subcellular Location:

  Nucleus . Binding to ubiquitin mediates localization to replication forks after UV-induced DNA damage. .

• Other Product Names:

  POLI; RAD30B; DNA polymerase iota; Eta2; RAD30 homolog B

• Gene ID (Human):

  11201

• SwissProt (Human):

  Q9UNA4