CD3EAP rabbit pAb

CD3EAP rabbit pAb

• Description: Caution: It is not known whether the so-called human ASE1 and human CAST proteins represent two sides of a single gene product with sharply different functional characteristics. Experiments done with the mouse homolog protein are in favor of an implication of this gene in rRNA transcription instead of T-cell receptor signaling. function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. function: Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex. miscellaneous: It is in an antisense orientation to and overlaps the gene of the DNA repair enzyme ERCC1. This gene overlap is conserved in mouse suggesting an important biologic function. PTM: Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes. PTM: Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Belongs to the eukaryotic RPA34 RNA polymerase subunit family. subcellular location: Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes. subunit: Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits. Interacts with TAF1A thereby associates with the SL1 complex. Interacts with UBTF. Interacts with POLR1E/PRAF1 through its N-terminal region (By similarity). Isoform 2 interacts with CD3E.
• Synonyms: CD3EAP; ASE1; CAST; PAF49; DNA-directed RNA polymerase I subunit RPA34; A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49
• Gene ID: 10849
• UniProt: O15446
• Cellular Locus: Nucleus, nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
• Host: Rabbit
• Species Reactivity: Human, Rat, Mouse,
• Immunogen: The antiserum was produced against synthesized peptide derived from human CD3EAP. AA range:441-490
• Clonality: Polyclonal
• Validated Applications: WB, IHC, IF, ELISA
• Stability: 1 year
• Concentration: 1 mg/mL
• Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Molecular Weight: 55kD
• Storage Conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.