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SPT16 rabbit pAb

CAT: 0855-ES3487-01Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0855-ES3487-01Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]
Description
Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009],
UniProt
Q9Y5B9
Swiss Prot
Q9Y5B9
Reactivity
Human; Mouse
Immunogen
The antiserum was produced against synthesized peptide derived from human SUPT16H. AA range:941-990
Clonality
Polyclonal
Source
Rabbit
Applications
WB; IHC; IF; ELISA
Concentration
1 mg/ml
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Molecular Weight
119kD
Storage Conditions
-20°C/1 year
Observed Molecular Weight
119kD
Fragment
IgG
Subcellular Location
Nucleus . Chromosome . Colocalizes with RNA polymerase II on chromatin. Recruited to actively transcribed loci.
Other Product Names
SUPT16H; FACT140; FACTP140; FACT complex subunit SPT16; Chromatin-specific transcription elongation factor 140 kDa subunit; FACT 140 kDa subunit; FACTp140; Facilitates chromatin transcription complex subunit SPT16; hSPT16
Gene ID (Human)
11198

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