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β-actin Mouse mAb

CAT: 0855-EM1031-02Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0855-EM1031-02Size:100 µL
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Background
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Description
β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.
Product Name Alternative
Actin antibody, b-actin antibody, beta-actin antibody
UniProt
P60709
Swiss Prot
P60709
Reactivity
Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Source
Mouse
Applications
WB, IHC, IF
Concentration
1mg/mL
Dilution
WB 1:5,000-10,000 IHC 1:200 IF 1:200
Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Molecular Weight
43kDa
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight
43kDa
Fragment
IgG1
Subcellular Location
Cytoplasm, Cytoskeleton
Other Product Names
Actin antibody, b-actin antibody, beta-actin antibody
Gene ID (Human)
60

UniProtKB · P60709

Actin, cytoplasmic 1

ACTB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P60709
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
P02570, P70514, P99021, Q11211, Q64316, Q75MN2, Q96B34, Q96HG5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationOxidationUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonNucleus

Disease

DeafnessDisease variantDystoniaIntellectual disability

Molecular function

Hydrolase

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