Human F-actin (Filamentous Actin) Microsample ELISA Kit

• Product Name Alternative:

  Fibrous Actin

• Reactivity:

  Human

• Field of Research:

  Signal transduction, Developmental science, Bone metabolism

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-actin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-actin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-actin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-actin in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  40 ng/mL

• Sample Type:

  Serum, plasma, tissue homogenates

• Detection Range:

  0.63 - 40 ng/mL

• Sensitivity:

  0.18 ng/mL