β-actin Mouse mAb

• Background:

  β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

• Description:

  β-actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, antibodies against β-actin are useful as loading controls for Western Blotting. However it should be noted that levels of β-actin may not be stable in certain cells. For example, expression of β-actin in adipose tissue is very low and therefore β-actin should not be used as loading control for these tissues.

• UniProt:

  P60709

• Swiss Prot:

  P60709

• Reactivity:

  Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep

• Immunogen:

  Synthetic Peptide

• Clonality:

  Monoclonal

• Source:

  Mouse

• Applications:

  WB, IHC, IF

• Concentration:

  1mg/mL

• Dilution:

  WB 1:5,000-10,000 IHC 1:200 IF 1:200

• Molecular Weight:

  43kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  43kDa

• Fragment:

  IgG1

• Subcellular Location:

  Cytoplasm, Cytoskeleton

• Other Product Names:

  Actin antibody, b-actin antibody, beta-actin antibody

• Gene ID (Human):

  60