IMAC Buffer (pH 8.0)

• Description:

  IMAC Buffer (pH 8.0)_x000D_ Catalog number: B2014673_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 250 mL_x000D_ Molecular Weight or Concentration: N/A_x000D_ Supplied as: Solution_x000D_ Applications: molecular tool for various biochemical applications_x000D_ Storage: 2-8°C_x000D_ Keywords: IMAC Buffer (pH 8.0)_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Martins S, Andrade J, Karmali A, Serralheiro ML. Screening of suitable immobilized metal chelates for adsorption of monoclonal antibodies against mutant amidase from Pseudomonas aeruginosa J Mol Recognit. 2006 Jul-Aug;19(4):340-7._x000D_ 2: Belew M, Yip TT, Andersson L, Ehrnström R. High-performance analytical applications of immobilized metal ion affinity chromatography Anal Biochem. 1987 Aug 1;164(2):457-65._x000D_ 3: Martins S, Karmali A, Andrade J, Serralheiro ML. Immobilized metal affinity chromatography of monoclonal immunoglobulin M against mutant amidase from Pseudomonas aeruginosa Mol Biotechnol. 2006 Jun;33(2):103-14._x000D_ 4: Zachariou M, Hearn MT. Adsorption and selectivity characteristics of several human serum proteins with immobilised hard Lewis metal ion-chelate adsorbents J Chromatogr A. 2000 Aug 18;890(1):95-116._x000D_ 5: Zachariou M, Hearn MT. Protein selectivity in immobilized metal affinity chromatography based on the surface accessibility of aspartic and glutamic acid residues J Protein Chem. 1995 Aug;14(6):419-30._x000D_ 6: do RosÃ��rio Freixo M, Karmali A, Arteiro JM. Production of polygalacturonase from Coriolus versicolor grown on tomato pomace and its chromatographic behaviour on immobilized metal chelates J Ind Microbiol Biotechnol. 2008 Jun;35(6):475-84._x000D_ 7: Huang Q, Chen L, Zeng L, Wan L, Li S, Lu X, Cheng J. [Evaluation of three in-vitro refolding methods for human-derived anti-CTLA4 scFv expressed in E. coli] Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2006 Apr;23(2):388-91._x000D_ 8: Thingholm TE, Jensen ON. Enrichment and characterization of phosphopeptides by immobilized metal affinity chromatography (IMAC) and mass spectrometry Methods Mol Biol. 2009;527:47-56, xi._x000D_ 9: Zhang A, Zhang C, Warikoo V, Forstrom J, Riske F. A modified IMAC method for the capture of target protein from mammalian cell culture harvest containing metal chelating species Biotechnol Bioeng. 2012 Mar;109(3):747-53._x000D_ 10: Barnouin KN, Hart SR, Thompson AJ, Okuyama M, Waterfield M, Cramer R. Enhanced phosphopeptide isolation by Fe(III)-IMAC using 1,1,1,3,3,3-hexafluoroisopropanol Proteomics. 2005 Nov;5(17):4376-88. _x000D_ _x000D_ Products Related to IMAC Buffer (pH 8.0) can be found at Chemicals

• Short Description:

  Catalog Number: B2014673 (250 mL)_x000D_ IMAC Buffer (pH 8.0) is a high quality ready-to-use IMAC Buffer (300 mM Imidazole in phosphate buffer) for elutiom of histidine-tagged proteins. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  35

• Length:

  8

• Width:

  8

• Height :

  8