FITC

CAT: 0804-HY-66019-01Size: 100 mgDry Ice: NoHazardous: No
CAT#:0804-HY-66019-01Size:100 mg
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Description
FITC (Fluorescein Isothiocyanate), is one of the green fluorescein derivatives widely used in biology. FITC has the characteristics of high absorptivity and excellent fluorescence quantum yield. The isothiocyanate group of FITC can be combined with amino, sulfhydryl, imidazole, tyrosyl, carbonyl and other groups on the protein, so as to achieve protein labeling including antibodies and lectins. In addition to its use as a protein marker, FITC can also be used as a fluorescent protein tracer to rapidly identify pathogens by labeling antibodies, or for microsequencing of proteins and peptides (HPLC) . The maximum excitation wavelength of FITC is 494 nm. Once excited, it fluoresces yellow-green at a maximum emission wavelength of 520 nm. In addition, FITC is also a hapten that can induce contact hypersensitivity (CHS) and induce an atopic dermatitis model[1][2][3][4].
CAS Number
[3326-32-7]
Product Name Alternative
Fluorescein 5-isothiocyanate
UNSPSC
12171500
Hazard Statement
H317, H334
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Inflammation/Immunology
Assay Protocol
https://www.medchemexpress.com/FITC.html
Purity
98.60
Solubility
DMSO : 62.5 mg/mL (ultrasonic)
Smiles
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4)C=C(O)C=C3)C5=C1C=C(N=C=S)C=C5
Molecular Formula
C21H11NO5S
Molecular Weight
389.38
Precautions
H317, H334
References & Citations
[1]Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12 (5) :3488-3494. Epub 2016 Oct 5.|[2]Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71|[3]Shigeno T, et al. Phthalate ester‐induced thymic stromal lymphopoietin mediates allergic dermatitis in mice[J]. Immunology, 2009, 128 (1pt2) : e849-e857.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light, stored under nitrogen)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported

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