Recombinant Human Interleukin-4 (IL4) (Active)

• Product Name Alternative:

  Interleukin-4; IL-4; B-cell stimulatory factor 1 (BSF-1) ; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra

• Abbreviation:

  Recombinant Human IL4 protein (Active)

• Gene Name:

  IL4

• UniProt:

  P05112

• Expression Region:

  25-153aa

• Organism:

  Homo sapiens (Human)

• Target Sequence:

  HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

• Tag:

  C-terminal 10xHis-tagged

• Type:

  Active Protein & In Stock Protein

• Source:

  Mammalian cell

• Field of Research:

  Immunology

• Relevance:

  Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses .

• Endotoxin:

  Less than 1.0 EU/μg as determined by LAL method.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Yes

• Bioactivity:

  The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is 1.864-4.949 ng/mL.

• Form:

  Lyophilized powder

• Buffer:

  Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

• Reconstitution:

  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

• Molecular Weight:

  16.3 kDa

• References & Citations:

  An IL-4 signalling axis in bone marrow drives pro-tumorigenic myelopoiesis. LaMarche N.M., Hegde S., Park M.D., Maier B.B., Troncoso L., Le Berichel J., Hamon P., Belabed M., Mattiuz R., Merad M. Nature 625:166-174 (2024)

• Storage Conditions:

  The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

• Protein Length:

  Full Length of Mature Protein