Human Interleukin-4 Recombinant

CAT: 0421-90193-ASize: 5 µgDry Ice: NoHazardous: No
CAT#:0421-90193-ASize:5 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Interleukin-4 is produced mainly by a subpopulation of activated T-cells (Th2) that are the biologically most active helper cells for B-cells and that also secrete IL-5 and IL-6. The biological activities of IL-4 are mediated by a specific receptor. The extracellular domain of the IL-4 receptor is related to the receptors for EPO, IL-6, and the beta chain of the IL2 receptor (CD124) . Two types of IL-4 receptor (IL-4R) exist: the type 1 receptor is a heterodimer consisting of CD132 and IL-4R-alpha. The type 2 receptor is a heterodimer consisting of IL-4Ralpha and IL-13Ralpha1. IL-4 enhances expression of MHC class 2 antigens on B-cells. It can promote their capacity to respond to other B-cell stimuli and to present antigens for T-cells. Pretreatment of macrophages with IL-4 prevents the production of IL1, TNF-alpha and prostaglandins in response to activation of the cells by bacterial endotoxins or IFN-gamma. IL-4 synergises with EPO and G-CSF in the generation of colonies containing granulocytes or erythroid progenitor cells in a colony formation assay.
Description
Recombinant human Interleukin-4 is a disulfide-linked monomer protein consisting of 130 amino acid residues, migrates as an approximately 15 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-4 mature chain was expressed inE. coli.
UniProt
P05112
Endotoxin
Endotoxin level was found to be < 0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Purity
≥ 95% by SDS-PAGE and HPLC
Format
Lyophilized protein
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/ml. This solution can then be diluted into other buffers.
Shipping Conditions
Gel Packs or Dry Ice
Storage Conditions
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Calculated Molecular Weight
15 kDa
Formulation
Lyophilized from a 0.2 µm filtered solution of 2.5% glycine, 0.5% sucrose, 0.01% Tween-80,5 mM glutamic acid, pH 4.5.

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