H-89

• Description:

  H-89 is a potent and selective inhibitor of cyclic AMP-dependent protein kinase (protein kinase A) with IC50 of 48 nM and has weak inhibition on PKG, PKC, Casein Kinase, and others kinases.

• UNSPSC:

  12352005

• Hazard Statement:

  H302, H312, H315, H319, H332, H335

• Target:

  Autophagy; PKA

• Type:

  Reference compound

• Related Pathways:

  Autophagy; Stem Cell/Wnt; TGF-beta/Smad

• Applications:

  Neuroscience-Neuromodulation

• Field of Research:

  Neurological Disease

• Assay Protocol:

  https://www.medchemexpress.com/H-89.html

• Purity:

  99.96

• Solubility:

  DMSO : 100 mg/mL (ultrasonic)

• Smiles:

  O=S(C1=CC=CC2=C1C=CN=C2)(NCCNC/C=C/C3=CC=C(Br)C=C3)=O

• Molecular Formula:

  C20H20BrN3O2S

• Molecular Weight:

  446.36

• Precautions:

  H302, H312, H315, H319, H332, H335

• References & Citations:

  [1]Chijiwa T, et al. Inhibition of forskolin-induced neurite outgrowth and protein phosphorylation by a newly synthesized selective inhibitor of cyclic AMP-dependent protein kinase, N-[2- (p-bromocinnamylamino) ethyl]-5-isoquinolinesulfonamide (H-89), of PC12D pheochromocytoma cells. J Biol Chem. 1990 Mar 25;265 (9) :5267-72.|[2]Blazev R, et al. Effects of the PKA inhibitor H-89 on excitation-contraction coupling in skinned and intact skeletal muscle fibres. J Muscle Res Cell Motil. 2001;22 (3) :277-86.|[3]Hosseini-Zare MS, et al. Effects of pentoxifylline and H-89 on epileptogenic activity of bucladesine in pentylenetetrazol-treated mice. Eur J Pharmacol. 2011 Nov 30;670 (2-3) :464-70.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  -20°C, 3 years; 4°C, 2 years (Powder)

• Scientific Category:

  Reference compound1

• Clinical Information:

  No Development Reported

• Citation 01:

  ACS Nano. 2025 Jul 1;19 (25) :22755-22766.|Acta Biochim Biophys Sin (Shanghai) . 2023 Nov 15;55 (12) :1855-1863.|Adv Sci (Weinh) . 2024 Jul 1:e2403640.|Adv Sci (Weinh) . 2025 Feb 7:e2413324.|Am J Physiol Cell Physiol. 2024 Jun 1;326 (6) :C1683-C1696.|Andrology. 2025 Jul 2.|Anim Nutr. 2022 Dec 31:13:185-196.|Ann Rheum Dis. 2025 Oct 28:S0003-4967 (25) 04428-0.|Aquac Rep. 2024 Jun, 36, 102110.|Aquac Res. 2020;00:1-10.|Arch Biochem Biophys. 2020 Jul 15;687:108375.|Biol Pharm Bull. 2024;47 (6) :1113-1118.|Biol Reprod. 2025 Sep 18:ioaf213.|Biomaterials. 2022 Dec:291:121902.|Biomed Pharmacother. 2022 Apr:148:112728.|bioRxiv. 2025 May 12.|bioRxiv. 2025 Nov 28.|BMC Biol. 2024 May 3;22 (1) :104.|BMC Genomics. 2024 Apr 3;25 (1) :337.|BMC Pharmacol Toxicol. 2023 Nov 20;24 (1) :63.|Bone. 2020 Jun;135:115326.|Brain Behav Immun. 2024 Jan:115:667-679.|Brain Behav Immun. 2024 Jul:119:36-50.|Cancer Cell Int. 2019 Sep 23;19:241.|Cell Commun Signal. 2021 Oct 11;19 (1) :103.|Cell Commun Signal. 2024 Jul 25;22 (1) :375.|Cell Death Dis. 2024 Sep 30;15 (9) :699.|Cell Death Dis. 2025 Aug 7;16 (1) :595.|Cell Death Discov. 2025 Apr 6;11 (1) :148.|Cell Metab. 2021 Mar 2;33 (3) :565-580.e7.|Cell Metab. 2021 Oct 5;33 (10) :2021-2039.e8.|Cell Mol Immunol. 2023 Feb;20 (2) :175-188.|Cell Rep. 2021 Sep 21;36 (12) :109726.|Cell Rep. 2022 Oct 18;41 (3) :111498.|Cells. 2023 Apr 6;12 (7) :1098.|Chem Biol Interact. 2024 Sep 2:111224.|Chem Sci. 2019 Dec 6;11 (4) :1132-1139.|Clin Transl Med. 2021 Jan;11 (1) :e266.|CNS Neurosci Ther. 2024 May;30 (5) :e14742.|Ecotoxicol Environ Saf. 2021 May:214:112005.|Endocrinology. 2023 Mar 13;164 (5) :bqad042.|Evid Based Complement Alternat Med. 2020 Dec 15:2020:8514926.|Fitoterapia. 2022 Nov:163:105336.|Free Radic Biol Med. 2024 May 31:222:72-84.|Front Oncol. 2021 Oct 6;11:738300.|Front Physiol. 2021 Oct 28;12:770430.|Front Physiol. 2022 Jan 5:12:736920.|Genes Dis. 2020 Nov 21;8 (6) :867-881.|Heart Surg Forum. 2024 Mar 17.|Heliyon. 2024 Sep 19;10 (19) :e37958.|Infect Immun. 2018 Dec 19;87 (1) . pii: e00508-1|Int Immunopharmacol. 2025 May 17:158:114885.|Int J Biol Macromol. 2025 Apr 26:143548.|Int J Biol Sci. 2024 Aug 19;20 (11) :4476-4495.|Int J Mol Sci. 2018 Oct 7;19 (10) . pii: E3053. |Int J Mol Sci. 2022 Dec 24;24 (1) :320.|Int J Mol Sci. 2022 Oct 20;23 (20) :12595.|Int J Mol Sci. 2023 Jan 16;24 (2) :1771.|Invest Ophthalmol Vis Sci. 2025 Jul 1;66 (9) :68.|iScience. 2021 Jul 8;24 (8) :102820.|iScience. 2025 Jan 21;28 (2) :111856.|J Adv Res. 2025 Apr 22:S2090-1232 (25) 00278-4.|J Agric Food Chem. 2023 Nov 1;71 (43) :16148-16159.|J Appl Biol Chem. 2025;68:26, 204‒211.|J Biol Chem. 2021 Jan-Jun:296:100644.|J Bone Miner Metab. 2021 Sep;39 (5) :748-756.|J Cell Mol Med. 2024 Apr;28 (8) :e18303.|J Exp Clin Cancer Res. 2021 Apr 8;40 (1) :122.|J Exp Clin Cancer Res. 2024 Apr 4;43 (1) :105.|J Gastroenterol Hepatol. 2025 Jul 31.|J Int Med Res. 2021 Mar;49 (3) :300060521999527.|J Integr Agric. 2023 Feb 24.|J Mater Sci Technol. 2023 Jun 30.|J Med Chem. 2025 Nov 13;68 (21) :23277-23299.|J Neuroinflammation. 2025 Oct 14;22 (1) :233.|J Neurophysiol. 2024 Jan 1;131 (1) :64-74.|J Neurosci. 2016 Oct 12;36 (41) :10560-10573.|J Orthop Surg Res. 2023 Nov 8;18 (1) :849.|J Periodontol. 2019 Sep;90 (9) :1002-1013.|Korean J Pain. 2023 Jul 1;36 (3) :335-346.|Life Sci. 2025 May 15:369:123553.|Microbiol Res. 2024 May 24:285:127779.|Mol Brain. 2021 Jul 19;14 (1) :116.|Mol Cell. 2023 Apr 6;83 (7) :1093-1108.e8.|Mol Neurobiol. 2025 Jan;62 (1) :366-385.|Mol Reprod Dev. 2022 May;89 (5-6) :256-268.|Nat Commun. 2022 Nov 4;13 (1) :6648.|Nat Commun. 2025 Jul 28;16 (1) :6942.|Neuro Oncol. 2024 Jan 5;26 (1) :137-152.|Neurogastroenterol Motil. 2020 Oct;32 (10) :1514-1528.|NPJ Biofilms Microbiomes. 2023 Feb 8;9 (1) :8.|Nutr Diabetes. 2024 Jul 23;14 (1) :56.|Nutrients. 2020 Jan 26;12 (2) :329.|Phytomedicine. 2025 Mar:138:156375.|Proteome Sci. 2023 Oct 11;21 (1) :16.|Psychoneuroendocrinology. 2020 Jul;117:104680.|Res Sq. 2024 Jul 15.|Res Sq. 2024 May 23.|Res Sq. 2025 Apr 08.|Research Square Preprint. 2020 Oct.|Research Square Preprint. 2023 Sep 13.|Sci Rep. 2025 Oct 16;15 (1) :36270.|Sci Signal. 2020 Nov 24;13 (659) :eaax0273.|Signal Transduct Target Ther. 2023 Aug 9;8 (1) :290.|Small Methods. 2025 Mar;9 (3) :e2401144.|SSRN. 2025 Apr 10.|SSRN. 2025 Mar 24.|Stem Cell Res Ther. 2020 Jan 3;11 (1) :2.|Theranostics. 2021 Mar 24;11 (12) :5650-5674.|Toxicol Lett. 2022 Apr 15:359:84-95.|Transl Psychiatry. 2025 Oct 6;15 (1) :366.|Acta Pharm Sin B. 2024 Mar;14 (3) :1222-1240.|Adv Sci (Weinh) . 2021 Dec;8 (24) :e2100808.|Adv Sci (Weinh) . 2025 Apr 28:e2409837.|Adv Sci (Weinh) . 2025 Sep 26:e02395.|Aging (Albany NY) . 2018 Jul 24;10 (7) :1722-1744. |Aging (Albany NY) . 2020 Jun 9;12 (11) :10951-10968.|Aging Cell. 2018 Jun;17 (3) :e12754. |AUTHOREA. 2025 May 27.|Biochem Biophys Res Commun. 2024 Apr 9:703:149689.|Biol Trace Elem Res. 2021 Jul;199 (7) :2687-2694.|bioRxiv. 2024 Feb 8.|bioRxiv. 2024 Oct 2:2024.09.30.615856.|bioRxiv. 2024 Oct 22:2024.10.18.619152.|Cell Commun Signal. 2022 Apr 12;20 (1) :52.|Cell Death Dis. 2025 Jan 20;16 (1) :31.|Elife. 2023 Jan 16:12:e81438.|Environ Health (Wash) . 2024 Aug 16;2 (11) :776-785.|Eur J Neurosci. 2025 Jul;62 (2) :e70198.|Eur J Pharmacol. 2024 Nov 6:985:177106.|Exp Cell Res. 2021 Feb 15;399 (2) :112482.|Exp Ther Med. 2022 Apr;23 (4) :248.|FASEB J. 2020 Oct;34 (10) :13376-13395.|Front Pharmacol. 2018 Apr 12:9:362.|Harvard Medical School LINCS LIBRARY|IJMPNP. 2019,5 (1) : 8-15.|Int Immunopharmacol. 2024 Feb 15:128:111490.|Int J Biol Sci. 2023 Apr 2;19 (7) :2006-2019.|J Agric Food Chem. 2025 Aug 13;73 (32) :20235-20253.|J Bone Miner Res. 2021 Aug;36 (8) :1605-1620.|J Cell Mol Med. 2020 Apr;24 (8) :4736-4747.|J Funct Foods. 2023 Dec, 111, 105902.|J Funct Foods. 2025 Aug.|Life Sci. 2022 Aug 15:303:120676.|Nat Commun. 2023 May 31;14 (1) :3159.|Nat Commun. 2025 Jul 1;16 (1) :5425.|Nat Metab. 2024 Apr;6 (4) :708-723.|PeerJ. 2022 Oct 25:10:e14192.|Peptides. 2022 Aug:154:170824.|Phytomedicine. 2025 Dec:149:157494.|Phytother Res. 2023 Apr;37 (4) :1330-1345.|Prog Neurobiol. 2021 Jul:202:102041.|Purinergic Signal. 2022 Sep;18 (3) :317-343.|Reprod Sci. 2023 Apr;30 (4) :1281-1290.|Research Square Preprint. 2024 Dec 04.|Sci Total Environ. 2022 Oct 10:842:156854.|Stem Cell Res Ther. 2020 May 19;11 (1) :182.|Theranostics. 2021 Jan 1;11 (6) :2612-2633.|Vet Microbiol. 2021 May:256:109061.

• CAS Number:

  [127243-85-0]