Anti-Hu CD1b APC

Anti-Hu CD1b APC

• Background: CD1b (also known as R1) together with CD1a and c, belongs to group 1 of CD1 antigens. These non-classical MHC-like glycoproteins serve as antigen-presenting molecules for a subset of T cells that responds to specific lipids and glycolipids found in the cell walls of bacterial pathogens or self-glycolipid antigens such as gangliosides, and they have also roles in antiviral immunity. The trafficking routes of the particular CD1 types differ and correspond to their ability to bind and present different groups of antigens. Besides non-peptide glycolipid antigen presentation to CD1-restricted T cells, CD1b has been implicated in thymocyte development.
• Specifications: The mouse monoclonal antibody SN13 (also known as K5-1B8) recognizes an extracellular epitope of CD1b, a 44 kDa type I glycoprotein associated with beta2-microglobulin. It is expressed on dendritic cells, Langerhans cells, thymocytes, and T acute lymphoblastic leukemia cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: A cell membrane antigen preparation that was isolated from normal human thymocytes
• Target Antigen: CD1b
• Isotype: Igg1 κ
• Clone: SN13
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Hayes SM, Knight KL: Group 1 CD1 genes in rabbit. J Immunol. 2001 Jan 1166(1):403-10., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=hayes+sm+knight+kl ,*Tentori L, Graziani G, Porcelli SA, Sugita M, Brenner MB, Madaio R, Bonmassar E, Giuliani A, Aquino A: Rifampin increases cytokine-induced expression of the CD1b molecule in human peripheral blood monocytes. Antimicrob Agents Chemother. 1998 Mar42(3):550-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/9517931 ,*Giuliani A, Prete SP, Graziani G, Aquino A, Balduzzi A, Sugita M, Brenner MB, Iona E, Fattorini L, Orefici G, Porcelli SA, Bonmassar E: Influence of Mycobacterium bovis bacillus Calmette Guérin on in vitro induction of CD1 molecules in human adherent mononuclear cells. Infect Immun. 2001 Dec69(12):7461-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/11705921,*Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., URL: ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.