Anti-Hu CD157 FITC

Anti-Hu CD157 FITC

• Background: CD157 (cADPr hydrolase 2) is a GPI-anchored ectoenzyme possessing ADP-ribosyl cyclase and cyclic ADP-ribose hydrolase activity. It uses NAD and cADP-ribose as substrates. CD157 is expressed on granulocytes, monocytes, macrophages, follicular dendritic cells, bone marrow stromal cells and human umbilical cord vein endothelial cells. In case of rheumatoid arthritis is expression is often higher and it is also differentially expressed in the myeloid leukemias. It may also have a signaling role.
• Specifications: The mouse monoclonal antibody SY11B5 recognizes CD157, an approximately 45 kDa GPI-anchored extracellular protein expressed mainly on monocytes, macrophages, granulocytes and bone marrow stromal cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Immunogen: Human CD157
• Target Antigen: CD157
• Isotype: Igg1 κ
• Clone: SY11B5
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: FITC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Arriga R, Caratelli S, Coppola A, Spagnoli GC, Venditti A, Amadori S, Lanzilli G, Lauro D, Palomba P, Sconocchia T, Del Principe MI, Maurillo L, Buccisano F, Capuani B, Ferrone S, Sconocchia G: Enhancement of anti-leukemia activity of NK cells in vitro and in vivo by inhibition of leukemia cell-induced NK cell damage. , URL: https://www.ncbi.nlm.nih.gov/pubmed/26655503,*Correia RP, Bento LC, Bortolucci AC, Alexandre AM, Vaz AD, Schimidell D, Pedro EC, Perin FS, Nozawa ST, Mendes CE, Barroso RS, Bacal NS: Technical advances in flow cytometry-based diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria. Einstein (Sao Paulo). 2016 Jul-Sep14(3):366-373., URL: https://www.ncbi.nlm.nih.gov/pubmed/27759825 ,*Sutherland DR, Richards SJ, Ortiz F, Nayyar R, Benko M, Marinov I, Illingworth A: CD71 improves delineation of PNH type III, PNH type II, and normal immature RBCS in patients with paroxysmal nocturnal hemoglobinuria. Cytometry B Clin Cytom. 2019 Nov 8. doi: 10.1002/cyto.b.21853. , URL: https://www.ncbi.nlm.nih.gov/pubmed/31705743,*Sutherland DR, Illingworth A, Marinov I, Ortiz F, Andreasen J, Payne D, Wallace PK, Keeney M: ICCS/ESCCA consensus guidelines to detect GPI-deficient cells in paroxysmal nocturnal hemoglobinuria (PNH) and related disorders part 2 - reagent selection and assay optimization for high-sensitivity testing. Cytometry B Clin Cytom. 2018 Jan94(1):23-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/29236353,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.