Biotinylated SARS-CoV-2 Spike Trimer Protein, His, Avitag™ (XBB/Omicron) (MALS verified)

• Background:

  It's been reported that coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  Biotinylated SARS-CoV-2 Spike Trimer, His, Avitag (XBB/Omicron) (SPN-C82Ew) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (T19I, LPP24-26del, A27S, V83A, G142D, Y144del, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P) . The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: XBB) . The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 139.5 kDa. The protein migrates as 180-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHD43416.1 (T19I, LPP24-26del, A27S, V83A, G142D, Y144del, H146Q, Q183E, V213E, G339H, R346T, L368I, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, V445P, G446S, N460K, S477N, T478K, E484A, F486S, F490S, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K, R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)

• Expression Region:

  Val 16 - Pro 1213

• Host:

  HEK293

• Target:

  Spike protein

• Conjugation:

  Biotin-labeled

• Tag:

  C-10xHis & C-Avi

• Source:

  SARS-CoV-2

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike Trimer, His,Avitag (XBB/Omicron) (Cat. No. SPN-C82Ew) with a linear range of 1-39 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  0.1 M Sodium citrate, pH5.5

• Additives:

  Trehalose

• Molecular Weight:

  139.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  25ug*1

• Species:

  SARS-CoV-2