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Human Nectin-2, NECTIN2 ELISA Kit

CAT: 1360-BPE005-01Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1360-BPE005-01Size:96 Tests
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Background
Cluster of Differentiation 112 (CD112), also known as poliovirus receptor related protein 2 (PVRL2 or PRR2), is a single-pass type I transmembrane glycoprotein belonging to the Immunoglobulin superfamily. CD112 protein also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and thus is involved in cell to cell spreading of these viruses. CD112 protein has been identified as the ligand for DNAM-1 (CD226), and the interaction of CD226/CD112 protein can induce NK cell- and CD8+ T cell-mediated cytotoxicity and cytokine secretion. CD112 has been regarded as a critical component in allergic reactions, and accordingly may function as a novel target for anti-allergic therapy.
Product Name Alternative
Nectin-2
Synonyms
HVEB; PRR2; PVRL2; CD112
Gene Name
NECTIN2
Gene ID
5819
UniProt
Q92692
Target
Nectin-2
Field of Research
Immunology, Microbiology
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 1000 pg/ml | 2.212 | 2.204 | 2.094 2.195 500 pg/ml | 1.26 | 1.238 | 1.128 1.215 250 pg/ml | 0.69 | 0.707 | 0.597 0.723 125 pg/ml | 0.4 | 0.408 | 0.298 0.415 62.5 pg/ml | 0.253 | 0.268 | 0.158 0.283 31.25 pg/ml | 0.187 | 0.189 | 0.079 0.19 15.625 pg/ml | 0.156 | 0.149 | 0.039 0.141 0 pg/ml | 0.112 | 0.11 | 0 0.107
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
15.625-1000 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 137 | 8.7 | 6.4 Intra-assay | 2 | 20 | 369 | 24.1 | 6.5 Intra-assay | 3 | 20 | 786 | 38.8 | 4.9 Inter-assay | 1 | 20 | 136 | 10.3 | 7.6 Inter-assay | 2 | 20 | 367 | 23 | 6.3 Inter-assay | 3 | 20 | 793 | 57.6 | 7.3
Sensitivity
4.04 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Human
Mass
57742

UniProtKB · Q92692

Nectin-2

NECT2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q92692
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NECTIN2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K5L5, O75455, Q6IBI6, Q96J29
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesionHost-virus interaction

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Host cell receptor for virus entryReceptor

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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