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Rat Nectin-1 ELISA Kit

CAT: 1360-BPE358-01Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:1360-BPE358-01Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Poliovirus receptor-related 1 (herpesvirus entry mediator C; nectin-1; CD111), also known as PVRL1 is a cell adhesion molecule belonging to the immunoglobulin superfamily that can bind to virion glycoprotein D (gD) to mediate entry of herpes simplex viruses (HSV) and pseudorabies virus (PRV) . CD111/Nectin-1/PVRL1 colocalizes with E-cadherin at adherens junctions in epithelial cells. The disruption of cell junctions can result in the redistribution of nectin-1. To determine whether disruption of junctions by calcium depletion influenced the susceptibility of epithelial cells to viral entry, Madin-Darby canine kidney cells expressing endogenous nectin-1 or transfected human nectin-1 were tested for the ability to bind soluble forms of viral gD and to be infected by HSV and PRV, before and after calcium depletion. It has been revealed that binding of HSV and PRV gD was localized to adherens junctions in cells maintained in normal medium but was distributed, along with nectin-1, over the entire cell surface after calcium depletion. Both the binding of gD and the fraction of cells that could be infected by HSV-1 and PRV were enhanced by calcium depletion. Taken together, CD111/Nectin-1/PVRL1 confined to adherens junctions in epithelial cells is not very accessible to virus, whereas dissociation of cell junctions releases nectin-1 to serve more efficiently as an entry recptor.
Product Name Alternative
Nectin-1
Synonyms
HVEC; PRR1; PVRL1; CD111; CLPED1; ED4; HIgR; MGC142031; OFC7; OTTHUMP00000232093; OTTHUMP00000232094; OTTHUMP00000232095; PRR; PVRRPVRR1; SK-12
Target
PVRL1
Field of Research
Microbiology, Neuroscience
Assay Type
Sandwich
Assay Protocol
Concentration | O.D. | Average | Corrected ------------------------------------------ 1500 pg/ml | 2.848 | 2.877 | 2.773 2.905 750 pg/ml | 1.688 | 1.617 | 1.513 1.546 375 pg/ml | 0.886 | 0.829 | 0.725 0.772 187.5 pg/ml | 0.457 | 0.453 | 0.349 0.448 93.75 pg/ml | 0.257 | 0.268 | 0.164 0.278 46.875 pg/ml | 0.171 | 0.174 | 0.07 0.176 23.4375 pg/ml | 0.134 | 0.13 | 0.026 0.126 0 pg/ml | 0.108 | 0.104 | 0 0.099
Assay Performance Time
3.5h
Sample Type
Serum, plasma, cell culture supernates
Detection Range
23.45-1500 pg/mL
Precision
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% ---------------------------------------------------------------- Intra-assay | 1 | 20 | 252 | 18 | 7.1 Intra-assay | 2 | 20 | 505 | 24.2 | 4.8 Intra-assay | 3 | 20 | 1010 | 54.7 | 5.4 Inter-assay | 1 | 20 | 250 | 22.9 | 9.2 Inter-assay | 2 | 20 | 506 | 37.3 | 7.4 Inter-assay | 3 | 20 | 1012 | 66.7 | 6.6
Sensitivity
9.2 pg/mL
Components
Components | Quantity --------------------- Pre-coated ELISA Plate | 12 x 8 well strips x 1 Standard Solution | 0.2mL x 1 vail Dilution Buffer | 20mL x 1 vail Stop Solution | 6mL x 1 vail Substrate Solution A | 12mL x 1 vail Substrate Solution B | 12mL x 1 vail Wash Buffer Concentrate (25x) | 20mL x 1 vail Detection Antibody Concentrate | 0.6mL x 1 vail
Storage Temperature
2-8°C
Species
Rat

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