MICROVUETMC5a EIA

The MICROVUE C5a Enzyme Immunoassay is a 96 well, direct-capture immunoassay for the measurement of C5a in human serum, plasma, and other biological or experimental samples. Under normal conditions, activation of the classical, alternative, or lectin complement pathways results in the formation of a C5 convertase multi-molecular enzyme capable of cleaving C5 to C5a and C5b. C5b is a key constituent of the Terminal Complement Complex and has a variety of functions in this role. C5a is a low molecular weight (approximately 9kD) protein fragment of 74 amino acids. C5a is rapidly metabolized by the serum enzyme carboxypeptidase to a more stable, less active, 73 amino acid form, C5a des-Arg. For convenience, both forms will be referred to as “C5a” for purposes of this documentation. The MICROVUE C5a assay, which provides a rapid, highly specific and quantitative procedure for measuring C5a levels, is designed for investigations into the role or status of terminal complement pathway activation in numerous research settings, and for monitoring the generation of C5a in vivo or in vitro. As the most potent of the complement anaphylatoxins, C5a has a host of biologic functions including mast cell degranulation, chemotaxis, leukosequestration, as well as cellular activation via binding to the C5a Receptor (C5aR or CD88) . Research has associated elevated levels of fluid phase and adsorbed C5a with hemo-incompatibility of some biomaterials, particularly in extracorporeal circuits. Research has also associated levels of C5a with pathogenesis of a variety of disease states including myocardial infarction, stroke, as well as vascular leak syndrome and associated kidney injury. The role of C5a in the pathogenesis of malaria20 and other infectious diseases, as well as sepsis, is likewise well documented.

The MICROVUE C5a Enzyme Immunoassay is for the measurement of C5a in human serum, plasma, and other biological or experimental samples.

None

2.5 hours

5

Serum 10 μL, EDTA or Citrated Plasma 20 μL

Serum 13.4–179.2 ng/mL, EDTA Plasma 0.37–74.3 ng/mL

0.01 ng/mL

96 well plate with 12 eight-well strips in a resealable foil pouch

Store the unopened kit at 2°C to 8°C. Refer to Product Insert for additional storage details.

2

7.1–13%