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MICROVUETMTRAP5b EIA

CAT: 1008-8033Size: 96 wells/testDry Ice: NoHazardous: No
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CAT#:1008-8033Size:96 wells/test
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TRAP5b (serum band 5 tartrate-resistant acid phosphatase, TRAcP 5b; EC 3.1.3.2) is a 35-37 kDa glycoprotein. TRAP5b is typically expressed in proportion to osteoclast activity and is secreted into circulation. As a result, serum TRAP5b is considered a potentially useful marker for bone resorption. The MICROVUE TRAP5b EIA detects the enzyme activity of bound TRAP5b in experimental samples.
Description
The MICROVUE TRAP5b Assay is an immunocapture enzyme assay for the determination of tartrate-resistant acid phosphatase isoform 5b (TRAcP 5b) in human serum and plasma.
Cross Reactivity
None
Assay Performance Time
2 hours
Standard
5
Sample Type
Serum/Heparin Plasma 50 μL
Sample Volume
Females (Ages 30–44) (premenopausal) 2.9±1.4 U/L or (Ages ≥50) (postmenopausal) 4.3±1.5 U/L, Males (Ages ≥20) 4.0±1.4 U/L
Limit Of Detection
0.24 U/L
Form
96 well plate with murine monoclonal anti-TRAP5b antibodies
Storage Conditions
Store unopened kit between 2°C to 8°C. Refer to manual for storing individual components
Controls
2
Inter Assay
2.0–3.0%
Intra Assay
1.9–2.2%

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