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OMNIMMP®RED fluorogenic substrate

CAT: 1106-BML-P277-0100Size: 100 µgDry Ice: NoHazardous: No
CAT#:1106-BML-P277-0100Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Certification
RUO
Sequence
TQ3-GABA-Pro-Cha-Abu-Smc-His-Ala-Dab (6′-TAMRA) -Ala-Lys-NH 2 [TQ3=quencher; GABA=4-aminobutyric acid; Cha=L-cyclohexylalanine; Abu=2-aminobutyric acid; Smc=S-methyl-L-cysteine; Dab=2,4-diaminobutyric acid; 6’-TAMRA=6’-tetramethylrhodamine]
Stability
As indicated on product label or CoA when stored as recommended.
Purity
≥90% (HPLC)
Solubility
Soluble in DMSO (50mM) .
Molecular Weight
1910.7
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
Emission at the red end of the spectrum avoids the interference at lower wavelengths often exhibited by screening compounds, and by substances commonly found in biological samples and tissue culture medium. MMP substrate peptides display poor aqueous solubility, often with K m s near or above their limits of solubility, making enzyme and inhibitor kinetics difficult. MMP K m s for OMNIMMP® RED substrate are below its solubility limit (~2μM in assay buffer), allowing for substrate concentrations higher than the K m , a condition generally desirable in endpoint assays. In addition to the efficient binding as exhibited by low K m s, OMNIMMP® RED is avidly cleaved by MMPs, with k cat / K m s in the range of 10 5 -10 7 M -1 sec -1 . The high k cat / K m s and the ultra-strong fluorescence of OMNIMMP® RED allow for substrate concentrations much lower than the K m , a condition generally desirable in inhibitor screening/kinetics assays. The following kinetic data [ k cat / K m (M -1 s -1) ; K m (μM) ] have been determined in-house. These are approximate only; customer should determine kinetics based on his/her assay conditions. MMP-1 (6.7x10 6 ; 0.48), MMP-2 (4.2x10 7 ; 0.38), MMP-3 (cleaves well; kinetics not yet determined), MMP-7 (1.6x10 7 ; 1.33), MMP-8 (1.6x10 7 ; 0.53), MMP-9 (2.3x10 7 ; 0.35), MMP-10 (cleaves well; kinetics not yet determined), MMP-11 (does not cleave), MMP-12 (cleaves well; kinetics not yet determined), MMP-13 (cleaves well; kinetics not yet determined), MMP-14 (cleaves well; kinetics not yet determined), MMP-19 (2.7x10 5 ; 0.50), MMP-20 (cleaves; kinetics not yet determined) .
Appearance
Lyophilized purple solid.
Applications Notes
Useful for inhibitor screening, kinetic analysis, and cellular activity assay.
Excitation
545nm
Emission
576nm; although the following Ex/Em can also be used: 540/590nm

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