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Caspase-3 fluorometric assay kit

CAT: 1106-ALX-850-216-KI01Size: 25 TestsDry Ice: NoHazardous: No
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CAT#:1106-ALX-850-216-KI01Size:25 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CPP32, Yama, Apopain
Certification
RUO
UniProt
P42574
Applications
Activity assay, Fluorescent detection
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
DEVD-AFC emits blue light (λmax = 400nm) ; upon cleavage of the substrate by caspase-3 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3 activity. Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125μl DEVD-AFC (1mM), 100μl DTT (1M) .

UniProtKB · P42574

Caspase-3

CASP3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P42574
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CASP3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
CASP-3
EC number
3.4.22.56
Processing
Precursor
Secondary accessions
A8K5M2, D3DP53, Q96AN1, Q96KP2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinS-nitrosylationUbl conjugationZymogen

Biological process

Apoptosis

Cellular component

Cytoplasm

Molecular function

HydrolaseProteaseThiol protease

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