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Caspase-2 fluorometric assay kit

CAT: 1106-ALX-850-214-KI01Size: 25 TestsDry Ice: NoHazardous: No
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CAT#:1106-ALX-850-214-KI01Size:25 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Certification
RUO
UniProt
P42575
Applications
Activity assay, Fluorescent detection
Shipping Conditions
Blue Ice
Storage Conditions
-20°C
Notes
VDVAD-AFC emits blue light (λmax = 400nm) ; upon cleavage of the substrate by caspase-2 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity. Biocompare Article: Keep an Eye on Apoptosis with Caspase Assays
Contents
25ml Cell Lysis Buffer, 2ml 2X Reaction Buffer, 125μl VDVAD-AFC Substrate (1mM), 100μl DTT.

UniProtKB · P42575

Caspase-2

CASP2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P42575
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CASP2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
CASP-2
EC number
3.4.22.55
Processing
Precursor
Secondary accessions
A8K5F9, D3DXD6, E9PDN0, P42576, Q59F21, Q7KZL6, Q86UJ3, Q9BUP7, Q9BZK9, Q9BZL0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinZymogen

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Disease

Disease variantIntellectual disabilityLissencephaly

Molecular function

HydrolaseProteaseThiol protease

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