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Recombinant Rat Fibronectin (Fn1), partial

CAT: 0399-CSB-MP008759RA2-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0399-CSB-MP008759RA2-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
FN; Fn1
Abbreviation
Recombinant Rat Fn1 protein, partial
Gene Name
Fn1
UniProt
P04937
Expression Region
25-404aa
Organism
Rattus norvegicus (Rat)
Target Sequence
TETGKSKRQAQQIVQPPSPVAVSQSKPGCFDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEPEETCFDKYTGNTYKVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDKWRRPHETGGYMLECLCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGNGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCVCTGNGRGEWKCERHVLQSASAGSGSFTDVRTAIYQPQTHPQPAPYGHCVTDSGVVYSVGMQWLKSQGDKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFHYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDH
Tag
C-terminal 10xHis-tagged
Type
Developed Protein
Source
Mammalian cell
Field of Research
Cell Biology
Relevance
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts
Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
45.0 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial

UniProtKB · P04937

Fibronectin

FINC_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P04937
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Fn1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
FN
EC number
—
Processing
Precursor
Secondary accessions
Q6LDX9
Protein keywords

Biological process

Acute phaseAngiogenesisCell adhesionCell shape

Coding sequence diversity

Alternative splicing

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinIsopeptide bondOxidationPhosphoproteinSulfation

Cellular component

Extracellular matrixSecreted

Molecular function

Heparin-binding

Domain

RepeatSignal