Recombinant Dog Mastin

CAT: 0399-CSB-YP325652DO-01Size: 20 µgDry Ice: NoHazardous: No

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CAT#:0399-CSB-YP325652DO-01Size:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

Mast cell protease 3; Mastocytoma protease

Abbreviation

Recombinant Dog Mastin protein

UniProt

P19236

Expression Region

31-280aa

Organism

Canis lupus familiaris (Dog) (Canis familiaris)

Target Sequence

IVGGCKVPARRYPWQVSLRFHGMGSGQWQHICGGSLIHPQWVLTAAHCVELEGLEAATLRVQVGQLRLYDHDQLCNVTEIIRHPNFNMSWYGWDSADIALLKLEAPLTLSEDVNLVSLPSPSLIVPPGMLCWVTGWGDIADHTPLPPPYHLQEVEVPIVGNRECNCHYQTILEQDDEVIKQDMLCAGSEGHDSCQMDSGGPLVCRWKCTWIQVGVVSWGYGCGYNLPGVYARVTSYVSWIHQHIPLSPGP

Tag

C-terminal 6xHis-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Others

Relevance

Trypsin-like serine protease. Has a preference for extended substrates with basic residues at the P1 position; Arg is preferred over Lys. Active towards calcitonin gene-related peptide and gelatin. Not active towards substance P, vasoactive intestinal peptide, type I collagen or azocasein

Purity

Greater than 95% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

29.3 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

UniProtKB · P19236

Mastin

TRYM_CANLF · Canis lupus familiaris

View on UniProt ↗

Primary accession: P19236
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: —
Protein existence: 1: Evidence at protein level
Organism: Canis lupus familiaris (Dog)
Taxonomy ID: 9615
Alternative names: —
EC number: 3.4.21.-
Processing: Precursor
Secondary accessions: Q66NX5, Q66NX6

Protein keywords

Cellular component

Cytoplasm

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

Signal

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