Mouse anti Bromodeoxyuridine / BrdU

Mouse anti Bromodeoxyuridine / BrdU

• Background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorpoRated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorpoRated BrdU with specific anti-BrdU monoclonal antibodies is an accuRate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorpoRated into the newly synthezised DNA of the S-phase cells and can thus provide an estimate for the fraction of cells in S-phase. Also dynamic prolifeRative information (such as the S-phase transit Rate and the potential doubling time) can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
• CAS Number: 9007-83-4
• Host: Mouse
• Isotype: IgG1
• Clone: IIB5
• Type: Primary Antibodies
• Source: IIB5 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse intraperitoneally immunized with BrdU conjugated to Bovine Serum albumin.
• Applications: Flow Cytometry, ICC, IHC (frozen), IHC (paraffin)
• Field of Research: Cancer, Cell cycle, Immunology, Neurobiology, Stem cell research
• Assay Principle: IIB5 is suitable for flow cytometry and immunohistochemistry on frozen and paraffin-embedded tissues. It can also be applied in immunocytochemical staining procedures on cultured cells. Optimal antibody dilution should be determined by titration; recommended range is 1:100 – 1:200 for flow cytometry, and for immunohistochemistry/immunocytochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
• Form: Each vial contains 100 µL 1 mg/mL purified monoclonal antibody in PBS containing 0,09% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• References & Citations: 1. Schutte, B., Reynders, M. M., van Assche, C. L., Hupperets, P. S., Bosman, F. T., and Blijham, G. H. (1987). An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry, Cytometry 8, 372-6. 2. _x000D_ 2. Tinnemans, M. M., Schutte, B., Lenders, M. H., Ten Velde, G. P., Ramaekers, F. C., and Blijham, G. H. (1993). Cytokinetic analysis of lung cancer by in vivo bromodeoxyuridine labelling, Br J Cancer 67, 1217-22. 3. _x000D_ 3. Schutte, B., Tinnemans M.M.F.J., Pijpers, G.F.P., Lenders, M.J.H. and Ramaekers, F. (1995). Three parameter flow cytometric analysis for simultaneous detection of cytokeratin, proliferation associated antigens and DNA content. Cytometry 21, 177-86. 4. _x000D_ 4. van Engeland, M., Kuijpers, H. J., Ramaekers, F. C., Reutelingsperger, C. P., and Schutte, B. (1997). Plasma membrane alterations and cytoskeletal changes in apoptosis. Exp Cell Res 235, 421-430. 5. _x000D_ 5. Schutte, B., Nieland, L., van Engeland, M., Henfling, M. E., Meijer, L., and Ramaekers, F. C. (1997). The effect of the cyclin-dependent kinase inhibitor olomoucine on cell cycle kinetics. Exp Cell Res 236, 4-15.
• Storage Conditions: The antibody is shipped at ambient temperature and may be stored at +4°C; For prolonged storage prepare appropriate aliquots and store at or below -20°C; Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2); Repeated thawing and freezing should be avoided; Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day; If a slight precipitation occurs upon storage, this should be removed by centrifugation; It will not affect the performance or the concentration of the product