Anti-Osteonectin/SPARC Antibody

• Description:

  Mouse Monoclonal Antibody specific to Osteonectin (SPARC)

• Product Name Alternative:

  Basement-membrane protein 40, BM-40, Osteonectin, ON, Secreted protein acidic and rich in cysteine

• Gene Name:

  SPARC

• NCBI Gene ID:

  SPARC

• UniProt:

  P09486

• Cellular Locus:

  Secreted, extracellular space, extracellular matrix, basement membrane

• Host:

  Mouse

• Reactivity:

  Human, Rabbit, Bovine, Porcine

• Immunogen:

  Bovine bone osteonectin (SPARC)

• Target Antigen:

  SPARC

• Target:

  Osteonectin/SPARC

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Type:

  Antibody

• Applications:

  WB, IHC, FACS

• Field of Research:

  Cell adhesion

• Purification Method:

  Purified by immunoaffinity chromatography

• Dilution:

  Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration).

• Format:

  Purified

• Form:

  Lyophilized

• Buffer:

  Phosphate Buffered Saline

• Function:

  Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

• Additionnal Information:

  ELISA: 1-10ug/ml with immobilized antigen
  
  Western Blot: 1-10ug/ml, reducing and non-reducing conditions.
  
  Immunohistochemistry: 1-10ug/ml, paraffin-embedded and frozen tissue sections.

• Storage Conditions:

  This product does not contain preservative. Lyophilized antibody is stable at 4°C for 2 years. Store the reconstituted solution (2. 0mg/ml) in aliquots at -20°C for one year or at 4°C for 6 months after adding 0.1% sodium azide. Dilutions of stock solution should not be stored. Avoid repeated freeze-thaw cycles.

• Specificity:

  This antibody reacts with human, bovine, rabbit, and porcine bone-derived and platelet derived osteonectin as well as with thrombin-stimulated platelets.

• Formulation:

  10 mM PBS, pH 7.4, 1% BSA, lyophilized

• Buffer pH:

  pH 7.4

• CAS Number:

  9007-83-4