SPARC/Osteonectin

• Description :

  SPARC/Osteonectin is a secreted, evolutionarily conserved collagen-binding glycoprotein that is involved in a variety of cellular activities. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC/Osteonectin and its related peptides bind to numerous proteins of the extracellular matrix (ECM), affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC/Osteonectin consists of three domains; an N-terminal acidic region that binds calcium ions with low affinity, a module containing two EF-hand motifs that bind calcium with high affinity, and a cysteine-rich follistatin-like domain. Recombinant human SPARC/Osteonectin is a glycoprotein containing 286 amino acids that migrates at an apparent MW of 43.7 kDa by SDS-PAGE analysis due to the effect of glycosylation.

• Synonyms :

  SPARC; ON

• NCBI Gene ID :

  6678

• UniProt :

  P09486

• Accession Number :

  NP_003109.1

• Accession Number mRNA :

  NM_003118.3

• Chromosomal Location :

  5q31.3-q32

• Reactivity :

  Human

• Cross Reactivity :

  Rat

• Sequence :

  APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI

• Endotoxin :

  < 0.1 ng/µg of protein (< 1EU/µg)

• Purity :

  > 98% by SDS-PAGE & HPLC analyses

• Bioactivity :

  Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3 cells, with an IC50 of 0.5-0.7 μg/ml.

• Length :

  286

• Form :

  Lyophilized

• Molecular Weight :

  43.7 kDa

• Host or Source :

  CHO cells