Anti-Osteocalcin Antibody

• Description:

  Mouse Monoclonal Antibody specific to Bovine Osteocalcin

• Product Name Alternative:

  Bone Gla protein, BGP, γ-carboxyglutamic acid-containing protein

• Gene Name:

  BGLAP

• NCBI Gene ID:

  BGLAP

• UniProt:

  P02818

• Cellular Locus:

  Secreted.

• Host:

  Mouse

• Reactivity:

  Human, Rat, Rabbit, Sheep, Goat, Bovine, Porcine

• Immunogen:

  Bovine osteocalcin

• Target Antigen:

  Osteocalcin

• Target:

  Osteocalcin

• Clonality:

  Monoclonal

• Isotype:

  IgG3

• Type:

  Antibody

• Applications:

  ELISA, IHC

• Field of Research:

  Hormones/Proteins

• Purification Method:

  Purified by immunoaffinity chromatography

• Dilution:

  Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration) then dilute further for applications in PBS or media that is identical to that used in the assay system.

• Format:

  Purified

• Form:

  Lyophilized

• Buffer:

  Tris

• Function:

  Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

• Additionnal Information:

  Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120.
  
  Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.
  
  Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.
  
  Endusers should determine optimal concentrations for their applications.

• Storage Conditions:

  Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.

• Specificity:

  Epitope within residues 21-31; human, bovine, rat, rabbit, pig, goat, and sheep.

• Formulation:

  20 mM TBS, pH7.5, containing 1.0% bovine serum albumin, lyophilized

• Target Background:

  Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.

• CAS Number:

  9007-83-4