Anti-eNOS Antibody

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme) . Several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (NOS-II), is Ca2+ independent and is expressed in a broad range of cell types, and two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (bNOS, NOS-I) identified in neurons and eNOS (ecNOS, NOS-III) identified in endothelial cells. Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation.

Our eNOS primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat eNOS and is antigen affinity purified. It is great for use in ELISA, WB, ICC, IHC.

Our eNOS primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat eNOS and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Endothelial Nitric Oxide Synthase, eNOS, ecNOS, NOS-III, NOS3, NOSIII

NOS3

P70313

Human, Mouse, and Rat

This antibody was generated from a recombinant mouse eNOS protein that included amino acids residues in the C-terminal region. This sequence is conserved in human and rat eNOS, and has low homology to other NOS family member

ENOS

Unconjugated

ELISA, WB, ICC, IHC

Cell Signaling

Antigen Affinity Purified

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

140

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Blue Ice

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Affinity purified rabbit serum

1:100

1:1000

The antibody detects a 140 kDa* protein corresponding to eNOS on SDS-PAGE immunoblots of human umbilical vein endothelial cells.

1:50

Western blots performed on each lot.

Rabbit

Liquid

IgG

Mouse

Quality Control: WB