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Recombinant Streptavidin-NC

CAT: 0793-32-4992-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0793-32-4992-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Source : Escherichia Coli. Recombinant Streptavidin-NC produced in E.Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 24kDa. Recombinant Streptavidin-NC not only binds to nitrocellulose membrane readily but also preserves the full biotin binding ability. Streptavidin is a tetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is wilyde used in molecular biology through its unique high affinity for the vitamin biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association of yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10**14) . The complexes are also extremely stable over a wide range of temperature and pH.
Purification
Greater than 94.0% as determined by SDS-PAGE.
Components
The sterile solution contains 10mM K2HPO4-KH2PO4, pH 7.3.
Storage Conditions
Streptavidin-NC although stable at 4°C for 3 weeks, should be stored below -18°C. Please prevent freeze thaw cycles.

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